The study concludes: ”These data suggest that anthocyanin supplementation (Medox®) may have a role in prevention or treatment of chronic inflammatory diseases by inhibition of NF-KappaB transactivation and deceased plasma concentration of pro-inflammatory chemokines, cytokines, and inflammatory mediators. R. Blomhoff et al, “Journal of Nutrition, August 2007, USA.

Anthocyanins Inhibit Nuclear Factor-kB Activation in Monocytes and Reduce Plasma Concentrations of Pro-Inflammatory Mediators in Healthy Adults

Anette Karlsen,4 Lars Retterstøl,6 Petter Laake,5 Ingvild Paur,4 Siv Kjølsrud-Bøhn,4 Leiv Sandvik,7 and Rune Blomhoff4*

4Department of Nutrition, and 5Department of Biostatistics, Institute of Basic Medical Sciences, University of Oslo, Oslo,Norway N-0316 and 6Department of Medical Genetics and 7Research Centre, Ullevaal University Hospital, Oslo, Norway N-0407


Abstract

The transcription factor nuclear factor-kB (NF-kB) is activated by oxidative stress and pro-inflammatory stimuli and controls the expression of numerous genes involved in the inflammatory response. Dampening NF-kB activation and thereby limiting the inflammatory response have been suggested as a potential strategy to prevent chronic inflammatory diseases. In cultured monocytes, anthocyanins isolated from bilberries and black currants (Medox) efficiently suppressed LPS-induced activation of NF-kB. Furthermore, we studied the effect of anthocyanin supplementation (Medox, 300 mg/d for 3 wk) in a parallel-designed, placebo-controlled clinical trial (n ¼ 120
men and women aged 40–74 y). Differences were observed in several NF-kB related inflammatory mediators in the Medox group compared to placebo. The changes in the NF-kBcontrolled pro-inflammatory chemokines IL-8, ‘‘regulated upon activation, normal T cell expressed and secreted,’’ (RANTES) and IFNa (an inducer of NF-kB activation) in the Medox group (45, 15, and 40% decreases from baseline, respectively) differed from those in the placebo group (20, 0, and 15% decreases from baseline, respectively) (P , 0.050). Similarly, changes in IL-4 and IL-13, 2 cytokines that mediate pro-inflammatory responses and induce NF-kB activation, in the Medox group (60 and 38% decreases from baseline, respectively) tended to differ from those in the placebo group (4 and 6% decreases) (P ¼ 0.056 and, P ¼ 0.089, respectively).These data suggest that anthocyanin supplementation may have a role in the prevention or treatment of chronic inflammatory diseases by inhibition of NF-kB transactivation and deceased plasma concentrations of pro-inflammatory chemokines, cytokines, and inflammatory mediators. J. Nutr. 137: 1951–1954, 2007.

4Department of Nutrition, and 5Department of Biostatistics, Institute of Basic Medical Sciences, University of Oslo, Oslo,Norway N-0316 and 6Department of Medical Genetics and 7Research Centre, Ullevaal University Hospital, Oslo, Norway N-0407